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<h1 style="color: #000 !important;">Abstract 20151117</h1>
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<p><strong style="background-color: initial;"><의공학연구소 정례세미나></strong></p><p><strong>연자 : 신용 교수 (융합의학과)</strong></p><p><strong>주제 : Recent advances in microfluidic systems for molecular diagnostic in clinical applications</strong></p><p><strong>일시 : 11월 17일 화요일 17:00~</strong></p><p><strong>장소 : 아산생명과학연구원 교육연구관 4층 회의실</strong></p><p><strong></strong></p><p> Miniaturized lab-on-a-chip (LOC) systems have been developed for genetic and epigenetic analyses in clinical applications because of advantages such as reduced sample size and reagent consumption, rapid processing speed, simplicity, and enhanced sensitivity. Despite tremendous efforts made towards developing LOC systems for use in the clinical setting, the development of LOC systems to analyze DNA methylation, which is an emerging epigenetic marker causing the abnormal silencing of genes including tumor suppressor genes, is still challenging because of the gold standard methods involving a bisulfite conversion step. Existing bisulfite conversion-based techniques are not suitable for clinical use due to their long processing time, labor intensiveness, and the purification steps involved. Here, we present a lab-on-achip system for DNA methylation analysis based on bisulfite conversion (LoMA-B), which couples a sample pre-processing module for on-chip bisulfite conversion and a label-free, real-time detection module for rapid analysis of DNA methylation status using an isothermal DNA amplification/detection technique. The methylation status of the RARβ gene in human genomic DNA extracted from MCF-7 cells was analyzed by the LoMA-B system within 80 min (except 16 h for sensor preparation) compared to conventional MS-PCR within 24 h. Furthermore, the LoMA-B system is highly sensitive and can detect as little as 1% methylated DNA in a methylated/unmethylated cell mixture. Therefore, the LoMA-B system is an efficient diagnostic tool for the simple, versatile, and quantitative evaluation of DNA methylation patterns for clinical applications. Furthermore, we have developed two novel techniques; a versatile Dimethyl adipimidate/Thin film based Sample processing (DTS) procedure as a DNA extraction technique without centrifugation and vortex steps. The DTS is useful for the extraction of DNA from a variety of sources, including cells, bacteria, blood, and urine in a single step. Specifically, the DTS procedure does not require a centrifuge and has improved time efficiency (30 min), affordability, and sensitivity in downstream analysis. We validated the DTS procedure for the extraction of DNA from human body fluids, as well as confirmed that the quality and quantity of the extracted DNA were sufficient to allow robust detection of genetic and epigenetic biomarkers in downstream analysis. Therefore, an integrated device of two novel techniques will be useful for analysis of genetic and epigenetic alteration of human disease related biomarkers with simplicity, rapidity, and low-cost.</p>
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