<div class="page photo" style=""> <article> <header style=" background-image:url(/imageLibrary/droplets.jpg); "> <div class="box"> <div class="intro" style="color: #000;"> <h1 style="color: #000 !important;">Abstract 20150901</h1> <p class="summary"></p> </div> </div> </header> <div class="main"> <div class="container"> <p class="byline"> </p> <p><strong style="background-color: initial;">&lt;의공학연구소 정례세미나&gt;</strong></p><p><strong>연자 : 정기석 박사 (의공학연구소)</strong></p><p><strong>주제 : In vitro models micrometastasis of cancer cells using a microfluidic device</strong></p><p><strong>일시 : 9월 1일 화요일 17:00~</strong></p><p><strong>장소 : 아산생명과학연구원 교육연구관 4층 회의실&nbsp;</strong></p><p><abstract></abstract></p><p>&nbsp;Microfluidic cell culture assays are versatile tools for studying endothelial cell migration, particularly angiogenesis. In this study, a spatiotemporally controlled <em>in vitro</em> model for mimicking the <em>in vivo</em> microenvironment is described to observe carcinoma cell micrometastasis. A blood vessel-mimicking channel was established, and pancreatic carcinoma cells were seeded into microwells containing biomimetic collagen extracellular matrix (ECM) within a microfluidic device. The device facilitated a successful demonstration of micrometastasis, including invasion of the cancer cells through ECM, transendothelial migration (TEM), colonization, and floating pancreatic carcinoma cells. During TEM, we found that round carcinoma cells aggregated into agglomerate before crossing the endothelial barrier, and underwent intravasation and colony formation. These changes in shape and aggregability facilitated disruption of the EC barrier, and accompanied by an increase in the local concentration of vascular endothelial cell growth factor. To the best of our knowledge, we first visualize that the carcinoma cells was floated into the cell culture medium flow in group, which can develop the circulating tumor cells.</p> </div> </div> </article> </div><!-- /page-->